. Multimode Reader Comparison Chart. . 2. Description. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Statistical analyses The statistical analyses were performed using Graph Prism version 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. We would like to show you a description here but the site won’t allow us. 0 out of 5 $ 17,999 $ 16,995. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. 1 or greater versions. Detection modalities are shown in Table 1-1. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The chamber contains. 5. SpectraMax M3, M4, and M5/M5e readers. The REF button has no valid meaning for. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. . User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. . 4. Exhibit with us {{>currencyLabel}} Back. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Weight. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. $4,499. I have been using the SpectraMax M5 since 2011. 0 out of 5 $ 15,999. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. 0200-0729. 1. . SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. D 1. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 2. used. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. 72 MB. Examples: “Press carefully to engage,” “ Do not. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. Step 2. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. com A. While they play an important role in generating data from a wide range of. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The M5 can read volumes down to 2uL with the. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. SpectraMax M5 and the Analyst GT. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 2 mm long, 86. 1) EarlyTox NucView 488 Assay (6. SpectraMax M5 device. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . . Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. The drawing shows a standard microplate of 15 mm height covered with a lid. In addition, the SpectraMaxMolecular Devices. Molecular Devices. . book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. Figure 1. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Customer Satisfaction is our number one goal All lab equipment in stock. Set the excitation at 531 nm and emission at 595 nm. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. Spectral Range. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. . Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. All reagents were brought to room temperature before use. Description 1. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. D 1 1. For SpectraMax L or LMax II, LMax II. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Bookings are free, but you will need to use iLab to book. 0 mm wide, and heights from 14 mm to 15 mm. 1. Wavelength Range. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. com A. 314 low lamp level warning. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The guide also covers the software features, the specifications, and the accessories of the systems. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Call 516-939-0888 to check current. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). Multimode Readers Comparison Chart. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. . When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 3. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The M5 can read volumes down to 2uL with the uMax Low. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. l. EarlyTox Live Cell Assay (6. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). It does not work as expected. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). These robust readers have been placed in labs from. 01) + 0. Spectranet customer portal. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Published Dec 16, 2020 | Updated Dec 16, 2020. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. D 1. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Set the excitation at 531 nm and emission at 595 nm. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 40266 Email: drugdiscoverytech@lifetech. With optimized reagents, option to operate the readers. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. the IMAP Platform and smoothly transition into high-throughput screening. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . 1. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The Spectr. This application note describes how to. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. acquired on a SpectraMax M5 (Molecular Devices). The following document is. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. 2. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SpectraMax Spectrofluorometers. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. . Add to Cart. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 3 or greater. Customer. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. . Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. The dual monochromator optics allow the widest range of applications to be utilized for bior. . Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 95. FlexStation® 3 Multi-Mode Microplate Reader. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For example, microplates containing different samples can be read using the312 gain calibration failed. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Proceed with the following steps to optimize the instrument: 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Example: [Drawer]. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. . The dynamic range of the assay was approximately 5 orders of magnitude. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. 5. Samples were analyzed in eight to sixteen wells per independent. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The. Sale. Refurbished. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. edu) to be added as a trained user in iLab. edu) to be added as a trained user in iLab. Flexible for 6-well to 384-well microplates. 304 signal level saturation. Description 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 6. Other technologies such as imaging. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. We would like to show you a description here but the site won’t allow us. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Find regional support. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 303 unable to cal dark current. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. The excitation and the emission wavelengths on. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. 304 signal level saturation. The corrected absorbance is within 5% of the. STAKMAX. There were 12 replicate wells per dilution in both 96-well and 384- well plates. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. Optimized for fluorescence microplate readers. In this. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Read fluorescence polarization at room temperature (RT, 19–25°C). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The following document is intended to demonstrate setup of this. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 3. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 316 grating motor driver faulty. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. 1321N1, a human astrocytoma cell line, expresses. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. , ~488 nm), has the optical. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 305 reference level saturation. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. SpectraMax Quant dsDNA Assay Kits. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Cavicide (Warehouse, Cat # 79300360) 6. The drawing shows a standard microplate of 15 mm height covered with a lid. Add to Cart. 2. Lift up the lid over the chamber to insert or remove a cuvette. 6. 9000-0756. 1. Download PDF versions of the SpectraMax M5 User Guides from this page. Introduction. 2. Fluorescent Polarization: Solid black plates, as above. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Small, unbound molecules rotate rapidly in solution (top), while large,. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. . Offer Ends In: 18 Days 19: 38: 21. Note: In this user guide, all references to SpectraMax Multi-Mode. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. KeySpan adapter for SoftMax Pro and SpectraMax readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. free shipping. view more . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). Monday to Friday 8am - 5pm GMT. The SpectraMax M5 Multi-Mode. UV/Vis Photometric Performance:. SpectraMax L Microplate Reader provides medium to high throughput. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. ; The top 3 importers of Spectramax are India with 365. Description 1. Table of Contents. Principle of the HTRF IP-One competitive binding assay. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The following document is intended to demonstrate setup of this instrument. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. Features. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 4. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. High tolerance for contaminants. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Fluorescence Microplate Reader Comparison Chart. These lab workhorses are used in drug discovery, research, bioassay validation, and. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Italic and boldface type are used for emphasis. . Cet appareil ISM est confomre à la norme NMB-001 du Canada. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 7. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. e. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. Click SoftMax Pro Software User Guide. 1. SoftMax Pro. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. View the Molecular Devices website. 4. . Step 1: Open the SoftMax Pro IMAP FP protocol. Table of Contents. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The chamber. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. Wash Buffer Concentrate was diluted 10-fold. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. UV/Vis Photometric Performance:. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Description. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M5 microplate reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. View the Molecular Devices website. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The following document is intended to demonstrate setup of this. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. For wavelengths in the UV range above 220 nm,. In this product video, see how the SpectraMax M5 quickly reads samples. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. 7°C (gradient heating design). No accessories are needed and SoftMax® Pro. 305 reference level saturation. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Maximum signal with lowest background. . EUROPE. NORTH AMERICA. These all-inclusive assay kits are ready to use in assay validation or screening. Microplate Reader Shelf. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Cavicide (Warehouse, Cat # 79300360) 6. Our absorbance plate readers feature our PathCheck Sensor. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. , ~488 nm), has the optical. 72 MB. Monday to Friday 7am - 5pm Pacific Time. 3. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Unit comes with software and computer. . type Clear.